Review



2 μg d1er  (Lonza)


Bioz Verified Symbol Lonza is a verified supplier
Bioz Manufacturer Symbol Lonza manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Lonza 2 μg d1er
    (A) Basal levels of [Ca 2+ ] i in control (C) and proband fibroblasts (P1, P2) and osteoblasts (P2). (B) Decreased Ca 2+ mobilization in TRIC-B deficient fibroblasts (red lines). ATP- and Ionomycin-stimulated Ca 2+ release, as well as the return to baseline levels, are decreased in proband (P2) cells. (C) Decreased Ca 2+ mobilization in TRIC-B deficient osteoblasts (red lines). (D) The intracellular Ca 2+ stores available for IP 3 R-mediated release are more rapidly depleted in Proband 1 fibroblasts (right) versus normal control cells (left). ATP-stimulated Ca 2+ release is abrogated within 10 minutes in proband cells compared to 30 minutes in control cells following inhibition of SERCA channels with thapsigargin (TG). (E) Measurement of ER luminal Ca 2+ using ER-localized Ca 2+ indicator. Each point represents the average of one measurement containing 1–7 cells. The difference between the steady-state and Ca 2+ -depleted FRET signal emitted by the <t>D1ER</t> chameleon class Ca 2+ sensor was equivalent in normal control and proband cells. (F) Quantitative RT-PCR in control (C) and proband (P1, P2, P3) cells. There is no significant difference in expression levels of SERCA2 ( ATP2A2 ) and IP3R1 ( ITPR1 ) in fibroblasts, but transcripts are significantly reduced in proband (P2) osteoblasts. (G) Immunoblots of control (C) and proband (P1, P2, P3) cell lysates demonstrate equivalent levels of SERCA2b and IP3R1 Ca 2+ channels. *, p < 0.05; **, p < 0.01.
    2 μg D1er, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2+%CE%BCg+d1er/pmc04956114-262-14-28?v=Lonza
    Average 90 stars, based on 1 article reviews
    2 μg d1er - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Absence of the ER Cation Channel TMEM38B /TRIC-B Disrupts Intracellular Calcium Homeostasis and Dysregulates Collagen Synthesis in Recessive Osteogenesis Imperfecta"

    Article Title: Absence of the ER Cation Channel TMEM38B /TRIC-B Disrupts Intracellular Calcium Homeostasis and Dysregulates Collagen Synthesis in Recessive Osteogenesis Imperfecta

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1006156

    (A) Basal levels of [Ca 2+ ] i in control (C) and proband fibroblasts (P1, P2) and osteoblasts (P2). (B) Decreased Ca 2+ mobilization in TRIC-B deficient fibroblasts (red lines). ATP- and Ionomycin-stimulated Ca 2+ release, as well as the return to baseline levels, are decreased in proband (P2) cells. (C) Decreased Ca 2+ mobilization in TRIC-B deficient osteoblasts (red lines). (D) The intracellular Ca 2+ stores available for IP 3 R-mediated release are more rapidly depleted in Proband 1 fibroblasts (right) versus normal control cells (left). ATP-stimulated Ca 2+ release is abrogated within 10 minutes in proband cells compared to 30 minutes in control cells following inhibition of SERCA channels with thapsigargin (TG). (E) Measurement of ER luminal Ca 2+ using ER-localized Ca 2+ indicator. Each point represents the average of one measurement containing 1–7 cells. The difference between the steady-state and Ca 2+ -depleted FRET signal emitted by the D1ER chameleon class Ca 2+ sensor was equivalent in normal control and proband cells. (F) Quantitative RT-PCR in control (C) and proband (P1, P2, P3) cells. There is no significant difference in expression levels of SERCA2 ( ATP2A2 ) and IP3R1 ( ITPR1 ) in fibroblasts, but transcripts are significantly reduced in proband (P2) osteoblasts. (G) Immunoblots of control (C) and proband (P1, P2, P3) cell lysates demonstrate equivalent levels of SERCA2b and IP3R1 Ca 2+ channels. *, p < 0.05; **, p < 0.01.
    Figure Legend Snippet: (A) Basal levels of [Ca 2+ ] i in control (C) and proband fibroblasts (P1, P2) and osteoblasts (P2). (B) Decreased Ca 2+ mobilization in TRIC-B deficient fibroblasts (red lines). ATP- and Ionomycin-stimulated Ca 2+ release, as well as the return to baseline levels, are decreased in proband (P2) cells. (C) Decreased Ca 2+ mobilization in TRIC-B deficient osteoblasts (red lines). (D) The intracellular Ca 2+ stores available for IP 3 R-mediated release are more rapidly depleted in Proband 1 fibroblasts (right) versus normal control cells (left). ATP-stimulated Ca 2+ release is abrogated within 10 minutes in proband cells compared to 30 minutes in control cells following inhibition of SERCA channels with thapsigargin (TG). (E) Measurement of ER luminal Ca 2+ using ER-localized Ca 2+ indicator. Each point represents the average of one measurement containing 1–7 cells. The difference between the steady-state and Ca 2+ -depleted FRET signal emitted by the D1ER chameleon class Ca 2+ sensor was equivalent in normal control and proband cells. (F) Quantitative RT-PCR in control (C) and proband (P1, P2, P3) cells. There is no significant difference in expression levels of SERCA2 ( ATP2A2 ) and IP3R1 ( ITPR1 ) in fibroblasts, but transcripts are significantly reduced in proband (P2) osteoblasts. (G) Immunoblots of control (C) and proband (P1, P2, P3) cell lysates demonstrate equivalent levels of SERCA2b and IP3R1 Ca 2+ channels. *, p < 0.05; **, p < 0.01.

    Techniques Used: Inhibition, Quantitative RT-PCR, Expressing, Western Blot



    Similar Products

    2 μg d1er  (Lonza)
    90
    Lonza 2 μg d1er
    (A) Basal levels of [Ca 2+ ] i in control (C) and proband fibroblasts (P1, P2) and osteoblasts (P2). (B) Decreased Ca 2+ mobilization in TRIC-B deficient fibroblasts (red lines). ATP- and Ionomycin-stimulated Ca 2+ release, as well as the return to baseline levels, are decreased in proband (P2) cells. (C) Decreased Ca 2+ mobilization in TRIC-B deficient osteoblasts (red lines). (D) The intracellular Ca 2+ stores available for IP 3 R-mediated release are more rapidly depleted in Proband 1 fibroblasts (right) versus normal control cells (left). ATP-stimulated Ca 2+ release is abrogated within 10 minutes in proband cells compared to 30 minutes in control cells following inhibition of SERCA channels with thapsigargin (TG). (E) Measurement of ER luminal Ca 2+ using ER-localized Ca 2+ indicator. Each point represents the average of one measurement containing 1–7 cells. The difference between the steady-state and Ca 2+ -depleted FRET signal emitted by the <t>D1ER</t> chameleon class Ca 2+ sensor was equivalent in normal control and proband cells. (F) Quantitative RT-PCR in control (C) and proband (P1, P2, P3) cells. There is no significant difference in expression levels of SERCA2 ( ATP2A2 ) and IP3R1 ( ITPR1 ) in fibroblasts, but transcripts are significantly reduced in proband (P2) osteoblasts. (G) Immunoblots of control (C) and proband (P1, P2, P3) cell lysates demonstrate equivalent levels of SERCA2b and IP3R1 Ca 2+ channels. *, p < 0.05; **, p < 0.01.
    2 μg D1er, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2+%CE%BCg+d1er/pmc04956114-262-14-28?v=Lonza
    Average 90 stars, based on 1 article reviews
    2 μg d1er - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Basal levels of [Ca 2+ ] i in control (C) and proband fibroblasts (P1, P2) and osteoblasts (P2). (B) Decreased Ca 2+ mobilization in TRIC-B deficient fibroblasts (red lines). ATP- and Ionomycin-stimulated Ca 2+ release, as well as the return to baseline levels, are decreased in proband (P2) cells. (C) Decreased Ca 2+ mobilization in TRIC-B deficient osteoblasts (red lines). (D) The intracellular Ca 2+ stores available for IP 3 R-mediated release are more rapidly depleted in Proband 1 fibroblasts (right) versus normal control cells (left). ATP-stimulated Ca 2+ release is abrogated within 10 minutes in proband cells compared to 30 minutes in control cells following inhibition of SERCA channels with thapsigargin (TG). (E) Measurement of ER luminal Ca 2+ using ER-localized Ca 2+ indicator. Each point represents the average of one measurement containing 1–7 cells. The difference between the steady-state and Ca 2+ -depleted FRET signal emitted by the D1ER chameleon class Ca 2+ sensor was equivalent in normal control and proband cells. (F) Quantitative RT-PCR in control (C) and proband (P1, P2, P3) cells. There is no significant difference in expression levels of SERCA2 ( ATP2A2 ) and IP3R1 ( ITPR1 ) in fibroblasts, but transcripts are significantly reduced in proband (P2) osteoblasts. (G) Immunoblots of control (C) and proband (P1, P2, P3) cell lysates demonstrate equivalent levels of SERCA2b and IP3R1 Ca 2+ channels. *, p < 0.05; **, p < 0.01.

    Journal: PLoS Genetics

    Article Title: Absence of the ER Cation Channel TMEM38B /TRIC-B Disrupts Intracellular Calcium Homeostasis and Dysregulates Collagen Synthesis in Recessive Osteogenesis Imperfecta

    doi: 10.1371/journal.pgen.1006156

    Figure Lengend Snippet: (A) Basal levels of [Ca 2+ ] i in control (C) and proband fibroblasts (P1, P2) and osteoblasts (P2). (B) Decreased Ca 2+ mobilization in TRIC-B deficient fibroblasts (red lines). ATP- and Ionomycin-stimulated Ca 2+ release, as well as the return to baseline levels, are decreased in proband (P2) cells. (C) Decreased Ca 2+ mobilization in TRIC-B deficient osteoblasts (red lines). (D) The intracellular Ca 2+ stores available for IP 3 R-mediated release are more rapidly depleted in Proband 1 fibroblasts (right) versus normal control cells (left). ATP-stimulated Ca 2+ release is abrogated within 10 minutes in proband cells compared to 30 minutes in control cells following inhibition of SERCA channels with thapsigargin (TG). (E) Measurement of ER luminal Ca 2+ using ER-localized Ca 2+ indicator. Each point represents the average of one measurement containing 1–7 cells. The difference between the steady-state and Ca 2+ -depleted FRET signal emitted by the D1ER chameleon class Ca 2+ sensor was equivalent in normal control and proband cells. (F) Quantitative RT-PCR in control (C) and proband (P1, P2, P3) cells. There is no significant difference in expression levels of SERCA2 ( ATP2A2 ) and IP3R1 ( ITPR1 ) in fibroblasts, but transcripts are significantly reduced in proband (P2) osteoblasts. (G) Immunoblots of control (C) and proband (P1, P2, P3) cell lysates demonstrate equivalent levels of SERCA2b and IP3R1 Ca 2+ channels. *, p < 0.05; **, p < 0.01.

    Article Snippet: Normal and proband fibroblasts (5 x 10 5 cells) were transfected with 2 μg D1ER or with 1 μg of pmaxGFP plasmid by electroporation using a 4D-Nucleofector system (Lonza), seeded into covered chamber slides and cultured at 37°C in 5% CO 2 for 48 hr.

    Techniques: Inhibition, Quantitative RT-PCR, Expressing, Western Blot